P-155 MicroRNA-155 (miR-155) expression profile and developmental characteristics of bovine early embryos are altered when cultured in group versus individually

Abstract Study question Culturing bovine preimplantation embryos in group versus individually has an impact on the expression profile of miR-155 and developmental characteristics. Summary answer MiR-155 was significantly greater its when were cultured groups singly. Developmental characteristics blastocysts similar regardless culture condition. What is known already MiRNAs are short non-coding RNAs that play important roles many biological pathways including embryogenesis potent effectors post-transcriptional gene silencing. It been estimated approximately 60% all proteins-coding genes regulated by miRNAs. involved early events embryogenesis; however, it not characterized this knowledge yet clinically translated. Existing literature indicates yields superior embryo development as can benefit from autocrine signaling paracrine communication but may be exposed to negative effects dying or delayed embryos. design, size, duration The study consisted two groups: zygotes those individually. Each sample consists 3 blastocysts. Droplet digital PCR (dd-PCR) used examine miR-155. Gene Set Enrichment Analysis (GSEA) miRNAs carried out identify functional Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enriched for targets one more Participants/materials, setting, methods Oocytes aspirated antral follicles ovaries matured vitro. Twenty-four hours post retrieval, oocytes inseminated with sperm bull. Presumptive either until blastocyst stage, Blastocyst collected purified RNA. KEGG GO Biological Process, Cellular Component Molecular Functions identified target genes. Results plotted according level enrichment Main results role chance Five hundred seventy-five retrieved 120 ovaries. Cleavage rate formation after IVF compared between There no significant difference among regards cleavage (69% vs. 54%) (42% vs.35%). ddPCR assessment showed (p = 0.002). highest miR-151 expressed 20 copies/sample. Every sample, conditions, had detectable level. interaction predicted clusters based Processes shows plays a DNA binding, transcription factor regulates affects cell adhesion. analysis revealed association steroidogenesis controls apoptosis pathways. Limitations, reasons caution number copies miRNA relatively low, which could suggest have at stage effectiveness depends relative binding site molecules. Blastocysts pooled morphological assessment. Wider implications findings offer novel mean assess embryonic fitness. Further validation complex designs required implement profiling diagnostic test selection. Group increased expression, possibly reflecting differences cellular behavior ones. Trial registration Not applicable